Knowhere is a mining colony controlled by the Collector, one of its point of interests are the Collector's Museum. It is a decapitated head of a Celestial.
Sif and Volstagg arrive at the museum, they are come to deliver the Aether to keep it safe, Tivan asks why they don't retain the Aether, Volstagg replied that's very dangerous to have two Infinity Stones in one place. The Asgardians leave and the Collector says: "One down five to go" implying that his mission is to take all Infinity Stones.
Once already escaped from prison, the Guardians are aimed at Knowhere to selling the Orb to Gamora's contact. Before meeting with the Collector, the Guardians have a discussion after drinking too much.
The majority of the guardians are going to the meeting, but one of them, Drax calls Ronan giving the whereabouts of Gamora. Ronan arrives with his troops destroying everything in its path to find the Orb.
Nebula gets the Orb and calls Ronan, who was fighting with Drax. Meanwhile, Peter calls Yondu and tells his whereabouts to save Gamora.
Thanos arrived on Knowhere to take Reality Stone from the Collector. The Guardians also arrived on Knowhere to prevent Thanos from taking the stone, only to discover that Thanos had already taken the Stone where he created an illusion to lure the Guardians into a trap so he would kidnap Gamora and interrogate her for the location of the Soul Stone.
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